Transfection of Escherichia coli Spheroplasts III. Facilitation of Transfection and Stabilization of Spheroplasts by Different Basic Polymers
نویسندگان
چکیده
منابع مشابه
Transfection of Escherichia coli Spheroplasts
The in vivo activitty of the recBC nuclease was assayed by transfection of isogenic rec+ and rec-spheroplasts with bacteriophage DNA of various origin and structure. The results indicate that the recBC nuclease can limit transfection at several stages during the production of an infective center; such limitations depend primarily on whether the DNA is in, or assumes, a nuclease-sensitive struct...
متن کاملTransfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction
Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. c...
متن کاملTransfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction
Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. c...
متن کاملFlagella of Escherichia coli spheroplasts.
Recently, Z. Vaituzis and R. N. Doetsch (J. Bacteriol. 89:1586, 1965) described the relationship between flagellation and motility in penicillin-induced spheroplasts of Salmonella typhimurium, and they concluded that the formation of flagella was dependent upon prior cell wall synthesis. This conclusion has now been confirmed with Escherichia coli (ATCC 13070), the results found being identical...
متن کاملTransfection of Escherichia coli and Salmonella typhimurium spheroplasts: host-controlled restriction of infective bacteriophage P22 deoxyribonucleic acid.
Under proper conditions, one infective center was obtained for 3 x 10(8) molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coli were transfected in the presence of 25 mug of protamine sulfate per ml. A 3- to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected ...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1973
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.12.4.741-747.1973